Aedes aegypti\u00a0<\/em>mosquito populations are particularly difficult to manage using traditional control techniques as they are diurnal mosquitoes that use cryptic breeding habitats, which makes their larvae difficult to find and remove. In addition, these mosquitoes are becoming increasingly resistant to insecticides. The Lee County Mosquito Control District (LCMCD), located in southwest Florida, USA, has been working to mitigate the public health threat posed by these mosquitoes since its inception in 1958. Increasing urbanization combined with increasing resistance to insecticides has led to a nearly ubiquitous spread of\u00a0Ae. aegypti<\/em>\u00a0throughout the county and created a need for the LCMD to identify alternative ways to combat this challenging species.<\/p>\n A new pilot project involving the\u00a0Sterile Insect Technique (SIT)<\/a>\u00a0is now being used to suppress populations of disease vector mosquitoes in Fort Myers\u00a0Florida. The SIT is an environmentally friendly pest control method which involves sterilizing male insects using radiation, before releasing them to mate with wild females, resulting in fewer or no offspring. The SIT pilot project is benefiting from extrabudgetary funding contributions from the IAEA\u2019s\u00a0Peaceful Uses Initiative\u00a0(PUI)<\/a>.<\/p>\n Rui Cardoso Pereira, Head of the Insect Pest Control at the Joint FAO\/IAEA Centre of Nuclear Techniques in food and Agriculture, says \u201cthe extrabudgetary PUI funds contributed by the USA have been instrumental for R&D based improvement of the SIT package for\u00a0Aedes<\/em>\u00a0mosquitoes and its further transfer to pilot projects in our Member States.\u201d\u00a0Aedes aegypti<\/em>\u00a0mosquitoes can spread diseases such as chikungunya, dengue, yellow fever and Zika, making them a significant threat to public health and therefore contributes to the attainment of\u00a0Sustainable Development Goal 3, good health<\/a>.<\/p>\n The SIT pilot project, which was initiated n the coastal city of Fort Myers, has already been tested out on Captiva Island in Florida, around 30 miles away, during a successful pilot project between 2020 and 2022. Male mosquitoes were mass-reared and sterilized before being released to mate with wild females. At the peak of releases, approximately 400 000 sterile males were released per week in Captiva Island. The releases led to a significant reduction of the population in the first year, 2020, and complete suppression in 2021 and 2022. Scientists were able to compare ecological indexes between Sanibel Island (the control area) and Captiva Island, where the sterile mosquitoes were released. Rachel Morreale, Manager of the Applied Science and Technologies Department at LCMCD, stated \u201cit was remarkable to see the impacts that our sterile male releases had on the population of\u00a0Ae. aegypti<\/em>\u00a0on Captiva.\u201d<\/p>\n Hurricane Ian devastated both Captiva and Sanibel islands completely in September 2022, making it impossible to access by car, and putting an end to the pilot project.<\/p>\n The damage caused was so significant that LCMCD determined that the best course of action would be to move the release programme to a new area on the mainland. Using lessons learned from the pilot project on Captiva Island, LCMCD collected baseline data to better inform their releases of sterile male\u00a0Ae. Aegypti<\/em>\u00a0in Fort Myers,\u00a0<\/em>which was initiated in February 2024. While the move to this new area was sooner than initially planned, the pilot project on Captiva Island allowed LCMCD to validate SIT as a component of an integrated mosquito management operation for the County.\u00a0Using the knowledge gained from mass-rearing, releases, and fieldwork, LCMCD is hopeful to have similar successful outcomes in Fort Myers and provide relief and protection to local residents.<\/p>\n According to David Hoel, Executive Director of LCMCD, \u201cthe unique attributes of this programme and technical expertise provided to us by the IAEA is enabling LCMCD to gain a foothold in suppression of this mosquito which is difficult at best to control by conventional mosquito control techniques and shows great promise for future prevention of mosquito-borne disease threat in Lee County, Florida.\u201d<\/p>\n![\"\"](\"https:\/\/www.fie.undef.edu.ar\/ceptm\/wp-content\/uploads\/2024\/06\/picture1.jpg\")