{"id":8263,"date":"2021-08-03T09:33:33","date_gmt":"2021-08-03T12:33:33","guid":{"rendered":"https:\/\/www.fie.undef.edu.ar\/ceptm\/?p=8263"},"modified":"2021-08-03T09:33:33","modified_gmt":"2021-08-03T12:33:33","slug":"metodo-de-verificacion-de-la-exposicion-a-los-agentes-nerviosos-de-novichok","status":"publish","type":"post","link":"https:\/\/www.fie.undef.edu.ar\/ceptm\/?p=8263","title":{"rendered":"M\u00e9todo de verificaci\u00f3n de la exposici\u00f3n a los agentes nerviosos de Novichok"},"content":{"rendered":"<p>Investigadores de TNO . CBRN Protection\u00a0de los Pa\u00edses Bajos, presentan un m\u00e9todo para verificaci\u00f3n por parte de la Organizaci\u00f3n para la Prohibici\u00f3n de Armas Qu\u00edmicas (OPAQ) y para investigaciones forenses peri\u00f3dicas.\u00a0Existe un m\u00e9todo bien aceptado para la evaluaci\u00f3n de la exposici\u00f3n a agentes nerviosos basado en el an\u00e1lisis espectrom\u00e9trico de masas de un nonap\u00e9ptido con el residuo de serina-198 modificado por el agente nervioso, pero este enfoque a\u00fan no se ha informado para la clase de agentes Novichok y requiere la disponibilidad de est\u00e1ndares de referencia.<\/p>\n<hr \/>\n<p><strong>Researchers from <a href=\"https:\/\/www.tno.nl\/en\/focus-areas\/defence-safety-security\/expertise-groups\/chemical-biological-radiological-and-nuclear-cbrn-protection\/\" target=\"_blank\" rel=\"noopener\">TNO\u2212CBRN Protection<\/a>, The Netherlands, present an important method for verification purposes by the Organisation for Prohibition of Chemical Weapons (OPCW) and for regular forensic investigations.<\/strong><\/p>\n<p>There is a well-accepted method for assessment of nerve agent exposure based on mass spectrometric analysis of a nonapeptide with the serine-198 residue modified by the nerve agent, but this approach has not yet been reported for the class of Novichok agents and requires the availability of reference standards.<\/p>\n<p>This study aims to first verify the utility of the nonapeptide method for the characterization of human plasma samples exposed in vitro to the Novichok nerve agents A-230, A-232, and A-234. The researchers then evaluate the possibility of identifying unknown exposures by applying precursor ion scanning in combination with high resolution mass spectrometry (HRMS).<\/p>\n<p>Precursor ion scanning, with a generic fragment ion (m\/z 778) of the nonapeptide, was used to pinpoint any modified nonapeptide, while HRMS was used for structural elucidation of the adduct moiety. Use of HRMS enabled differentiation between adducts of agents with similar molecular masses.<\/p>\n<p>A new unique feature that could be exploited for Novichok agent nonapeptide analysis was that the modification was released from the peptide during fragmentation in the mass spectrometer and was detected in the low-mass region of the mass spectrum. This low-mass region was extremely informative and contributed to the assignment of the structure of the particular agent used, which is especially important in case no reference materials are available.<\/p>\n<p><strong>Fuente:<\/strong> <a href=\"https:\/\/globalbiodefense.com\/2021\/07\/14\/method-for-verification-of-exposure-to-novichok-nerve-agents\/\" target=\"_blank\" rel=\"noopener\"><em>https:\/\/globalbiodefense.com<\/em><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Investigadores de TNO . CBRN Protection\u00a0de los Pa\u00edses Bajos, presentan un m\u00e9todo para verificaci\u00f3n por parte de la Organizaci\u00f3n para la Prohibici\u00f3n de Armas Qu\u00edmicas&hellip; <\/p>\n","protected":false},"author":1,"featured_media":8264,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[24],"tags":[],"_links":{"self":[{"href":"https:\/\/www.fie.undef.edu.ar\/ceptm\/index.php?rest_route=\/wp\/v2\/posts\/8263"}],"collection":[{"href":"https:\/\/www.fie.undef.edu.ar\/ceptm\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.fie.undef.edu.ar\/ceptm\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.fie.undef.edu.ar\/ceptm\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.fie.undef.edu.ar\/ceptm\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8263"}],"version-history":[{"count":1,"href":"https:\/\/www.fie.undef.edu.ar\/ceptm\/index.php?rest_route=\/wp\/v2\/posts\/8263\/revisions"}],"predecessor-version":[{"id":8265,"href":"https:\/\/www.fie.undef.edu.ar\/ceptm\/index.php?rest_route=\/wp\/v2\/posts\/8263\/revisions\/8265"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.fie.undef.edu.ar\/ceptm\/index.php?rest_route=\/wp\/v2\/media\/8264"}],"wp:attachment":[{"href":"https:\/\/www.fie.undef.edu.ar\/ceptm\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8263"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.fie.undef.edu.ar\/ceptm\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8263"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.fie.undef.edu.ar\/ceptm\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8263"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}